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Silicone based SPE Bond Elut Certfy II hybrid non-polar and strong anion exchange column
*Ideal selection of non-polar and anionic compounds * Optimization of acidic drug analysis * Dual mode, non-polar, and strong anion exchange
Product details
Bond Elut Certification II is a product specifically designed for forensic applications to rapidly and effectively extract acidic drugs and metabolites from urine and other biological matrices. Certification II is a mixed mode extraction column with non-polar C8 and strong anion exchange (SAX) functional groups. It has been used for optimizing the extraction of acidic drugs, such as 11-n-D9-tetrahydrocannabinoic acid, salicylic acid, ibuprofen, acetaminophen, and other compounds with both non-polar and anionic characteristics.
Common matrix |
Main extraction mechanism |
Type of compound |
Urine, plasma, saliva, blood, and biological fluids |
Non polar and strong anion exchange |
Acidic drug, acid abuse drug |
Bond Elut Certify Ⅱ
Explanation |
unit |
40 μ m filler particle size |
120 μ m filler particle size |
Large Capacity Column Tube (LRC) | |||
100 mg，10 mL |
50/pack |
| |
200 mg，0 mL |
50/pack |
||
Straight pipe column | |||
50 mg，3 mL |
50/pack |
| |
100 mg，1 mL |
100 per pack |
102818C |
|
200 mg，3 mL |
50/pack |
||
500 mg，6 mL |
30/pack |
||
1 g，6 mL |
30/pack |
||
Other types | |||
Prospekt Column, 800 Series |
96/pack |
| |
utilizeBond Elut Certification II extracts THC-COOH from human urine
Adsorbent activation: |
100% methanol, then 0.1M acetic acid buffer, pH 7.0 |
Sample processing: |
Add 300 μ L of 10M potassium hydroxide and matrix standard sample to 6mL of urine sample. Vortex oscillation, hydrolysis at 60 ℃ for 15 minutes, and then cooling. Add 165 μ L of glacial acetic acid and 2mL of 95% 0.1M acetic acid buffer/5% methanol at pH 7.0. Adjust the pH of the sample to between 4.5 and 6.5 using glacial acetic acid, and pass it through the adsorbent at a flow rate of<4mL/min |
Rinse off interfering substances: |
1. 10mL 50:50 H2O/MeOH solution, then dry the adsorbent under vacuum for 10 minutes; 2. 2mL EtOAc, dry for 0.5 minutes; |
Elution of analytes: |
2mL of 25% EtOAc/75% hexane solution containing 1% AcOH; |
Silicone substrate: irregular shape of 40 μ m or 120 μ m, acid washed treatment, 60 μ m? Average pore size
● Bonded phase: mixed mode adsorbent: octyl (C8) and quaternary amino (SAX)
End group sealing: No
Typical carbon load: 8.6%
Main retention mechanism: dual mode, non-polar and strong anion exchange
Application sample types: urine, biological fluid samples
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